NEW PATENT WO/2021/180078 METHOD FOR PREPARING CHITOSAN USING SNOW CRAB SHELLS

 NEW PATENT

WO/2021/180078 METHOD FOR PREPARING CHITOSAN USING SNOW CRAB SHELLS
https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2021180078&_gid=202137

SHANDONG MEIJIA GROUP CO.LTD

Take 200g of fresh snow crab shells, that is, leftovers produced by fresh snow crabs just after aquatic processing in the previous process. After crushing, add 1000g of citric acid solution with a concentration of 5% by mass, soak for 15 hours, filter with gauze, and use tap water or The crab shell is washed with pure water to obtain a decalcified crab shell. The washing residue and the filtrate are combined to obtain a mixture. The mixture is filtered through a plate and frame, and the filter residue is dried to obtain calcium citrate. Add 1000g of 5% citric acid solution and repeat the above steps once to obtain decalcified crab shells; add 1000g of water to the decalcified crab shells, add 2g of flavor enzymes, keep them at 49°C for 3 hours, inactivate the enzymes, filter, and obtain The filtrate is concentrated and spray-dried to obtain crab protein powder. The obtained crab shells are added to 400 g of 10% citric acid solution, soaked for 6 hours, filtered with gauze, and the resulting filter residue is washed and dried to obtain chitin. The obtained filtrate is recovered for subsequent processing. For use, add 55% KOH solution (containing 0.1% potassium acetate) to the obtained chitin, where the mass percentage of chitin and KOH solution is 1:5, at 90°C, keep for 6 hours, filter after cooling, wash, and recover the filtrate and The washing liquid is used again after subsequent treatment. The washed filter residue is irradiated with ultraviolet rays for 12 hours, and 24.2 g of chitosan is obtained after drying.

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